Phosphatidylinositol Hydrolysis by Glycosylphosphatidylinositol Phospholipase C
نویسندگان
چکیده
منابع مشابه
Phospholipase C - mediated Hydrolysis of Phosphatidylcholine
We have investigated the effect of cis-diamminedichloroplatinum(II) (CDDP) on signal transduction pathways. CDDP treatment did not cause any change in the binding of 1H1phorbol dibutyrate to PC-9 (human lung adenocarcinoma cell line) cells, a measure of protein kinase C activation. However, 2-h CDDP treatment (20 gg/ml) caused 200% increase in 1,2-sn-diacylglycerol (DAG) production and 50% decr...
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Trypanosoma brucei glycosylphosphatidylinositol phospholipase C (GPIPLC) is expressed in the bloodstream stage of the life cycle, but not in the procyclic form. It is capable of hydrolyzing GPI-anchored proteins and phosphatidylinositol (PI) in vitro. Several roles have been proposed for GPIPLC in vivo, in the release of variant surface glycoprotein during differentiation or in the regulation o...
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The infectious stages of Trypanosoma brucei are covered by a homogenous coat consisting of 10 million molecules of a glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) (for a review, see [1]). The bloodstream forms express a phospholipase, the GPI-specific phospholipase C (GPIPLC), which has a predilection for cleaving the GPI anchor of cell-surface VSG when trypanos...
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A novel phospholipase C was obtained from Bacillus cereus in 1976 with the specificity to act upon phosphatidylinositol (see separate webpage) to generate diacylglycerol and inositol phosphate. When this was tested with tissues a year or two later, it was found to release in addition a variety of proteins including 5’-nucleotidase and erythrocyte acetylcholinesterase. It was apparent that these...
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Generation of a phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3] gradient within the plasma membrane is important for cell polarization and chemotaxis in many eukaryotic cells. The gradient is produced by the combined activity of phosphatidylinositol 3-kinase (PI3K) to increase PI(3,4,5)P3 on the membrane nearest the polarizing signal and PI(3,4,5)P3 dephosphorylation by phosphatase and t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.26.15533